Extended Data Fig. 5: Multiple sequence alignments and AlphaFold-predicted structures of Siglecs.
From: Carbon nanotube recognition by human Siglec-14 provokes inflammation
a, Amino acid sequences of Siglec V-set Ig-like domain were aligned with CLUSTALW. Red solid squares indicate aromatic clusters. Red dot squares indicate an incomplete aromatic cluster. Numbers indicate amino acid positions of the N-terminal V-set Ig-like domain without signal sequence of Siglec-7. b, A phylogenetic tree was generated with CLUSTALW. Numbers indicate branch lengths. c, Protein structures of Siglecs were predicted by AlphaFold. The extracellular loops are surrounded by black dot squares. Aromatic residues in the extracellular loops are indicated by black arrowheads.
