Extended Data Fig. 6: Generation of anti-Siglec-5/14 neutralizing mAbs. | Nature Nanotechnology

Extended Data Fig. 6: Generation of anti-Siglec-5/14 neutralizing mAbs.

From: Carbon nanotube recognition by human Siglec-14 provokes inflammation

Extended Data Fig. 6: Generation of anti-Siglec-5/14 neutralizing mAbs.The alternative text for this image may have been generated using AI.

a, Parental Jurkat.EcoR cells, and Jurkat.EcoR cells stably expressing Siglec-5 or Siglec-14 were pretreated with control mouse IgG1 (cIg), or anti-Siglec-5/14 neutralizing mAbs, SY1 and SY2 (10 μg/ml each), and then these cells were cultured with MWCNTs (30 μg/ml) for 30 min. MWCNT recognition was analyzed as in Fig. 1c. b, The indicated Jurkat.EcoR cells and NIH-3T3 cells were stained with biotinylated cIg, SY1, or SY2, followed by PE-streptavidin. Cells were analyzed by flow cytometry.

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