Extended Data Fig. 7: Effect of sialidase on Siglec-14 responses to MWCNTs.
From: Carbon nanotube recognition by human Siglec-14 provokes inflammation
a, Siglec-14/THP-1 cells were treated with the indicated activity of sialidase for 1 hr and were then stained with indicated mAbs. Desialylation was assessed by flow cytometry using anti-sialylated CD43 mAb (clone 1G10). b, Cells and MWCNTs (30 μg/ml) were each pretreated with sialidase as in a, and then were combined and cultured for 30 min. MWCNT binding was analyzed by flow cytometry. c, MWCNTs (30 μg/ml) and PMA-primed cells were each pretreated with sialidase as in a, and then were combined and cultured for 5 hr in serum-free medium containing 1% BSA. IL-1β secretion was analyzed by ELISA. Data are shown as mean ± SD (n = 3). ***p < 0.001, two-way ANOVA with Tukey-Kramer test.
