Extended Data Fig. 8: Siglec-14 recognize SWCNTs resulting in induction of IL-8, but not IL-1β, secretion.
From: Carbon nanotube recognition by human Siglec-14 provokes inflammation
a, b, Size and shape of MWCNTs and SWCNTs were analyzed by transmission electron microscopy (a), and by light microscopy in 0.5% BSA/PBS (b). c, MWCNTs and SWCNTs were stained with cIg (black lines) or Siglec-14-Ig (blue lines), followed by AF647-anti-mouse IgG in 0.5% BSA/PBS. Siglec-14-Ig binding was analyzed by flow cytometry. d, Indicated THP-1 cells were primed with PMA (0.5 μM) for 12 hr and were treated with the indicated dose of MWCNTs or SWCNTs for 5 hr. IL-1β secretion was analyzed by ELISA. Data are shown as mean ± SD (n = 3). e, Indicated THP-1 cells were stimulated with the indicated dose of MWCNTs or SWCNTs for 5 hr. IL-8 secretion was analyzed by ELISA. See also Method section. Data are shown as mean ± SD (n = 3). **p = 0.0364, ***p < 0.01, two-way ANOVA with Tukey-Kramer test to compare each mean with every other mean.
