Fig. 5: QolorEX RCA analytical sensitivity and selectivity in human saliva. | Nature Nanotechnology

Fig. 5: QolorEX RCA analytical sensitivity and selectivity in human saliva.

From: Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens

Fig. 5: QolorEX RCA analytical sensitivity and selectivity in human saliva.

a, Plasmonic enhanced colorimetric detection of SARS-CoV-2 variants of concern. The colour palette represents the detected colorimetric read-out for different concentrations of SARS-CoV-2 cDNA (y axis) versus time (x axis). SARS-CoV-2 detection is shown by the rapid colour change from fuchsia to pink and then orange/yellow. b, The corresponding QolorEX signal shows an overall increase in QolorEX signal output over time. c, SARS-CoV-2 genomic map indicating the target sequences used for this study. E, envelope gene; N, nucleocapsid gene. d, The magnitude of QolorEXRCA-WT signal for different concentrations of SARS-CoV-2 WT cDNA in saliva (N = 40 per sample condition). e, The QolorEXRCA-WT signal as a linear function of SARS-CoV-2 WT cDNA concentration in saliva (\(R_{{\mathrm{saliva}}}^2 = 0.99\)). f, Quantification of the QolorEXRCA-WT selective detection of the SARS-CoV-2 WT cDNA versus the cDNA of different SARS-CoV-2 variants in saliva (N = 60, F value = 2,005.41485, Fprob = 2.50961 × 10–122). g, The magnitude of the QolorEXRCA-Delta signal for different concentrations of SARS-CoV-2 Delta variant cDNA in saliva (N = 40 per sample condition). h, The QolorEXRCA-Delta signal as a linear function of SARS-CoV-2 Delta variant cDNA concentration in saliva (\(R_{{\mathrm{saliva}}}^2 = 0.96\)). i, Quantification of the QolorEXRCA-Delta selective detection of the SARS-CoV-2 Delta variant cDNA versus the cDNA of different SARS-CoV-2 variants in saliva (N = 60, F value = 107.98492, Fprob = 2.2123 × 10–31). j, The magnitude of the QolorEXRCA-Omicron signal for different concentrations of SARS-CoV-2 Omicron variant cDNA in saliva (N = 40 per sample condition). k, The QolorEXRCA-Omicron signal as a linear function of SARS-CoV-2 Omicron variant cDNA concentration in saliva (\(R_{{\mathrm{saliva}}}^2 = 0.97\)). l, Quantification of the QolorEXRCA-Omicron selective detection of the SARS-CoV-2 Omicron variant cDNA versus the cDNA of different SARS-CoV-2 variants in saliva (N = 60, F value = 193.02268, Fprob = 3.33077 × 10–45). Quantification of the data shows mean values ± standard error of the mean with a coefficient of 1.5 for all panels. ***P < 0.001 as determined by one-way ANOVA with post hoc Tukey’s test.

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