Fig. 1: Formation of capsid-coated DNA origami structures.
a, CPs are isolated from native CCMV (left) and complexed with different DNA origami shapes, resulting in a coating (middle) whose properties are determined by the origami structure. The assembly is driven by electrostatic (positively charged amino acids in the N-terminus marked in red) and protein–protein interactions (right). A second protein layer can develop on top of the first one due to electrostatic interactions between the N-terminus and the negatively charged parts of the CP surface. b, Negative-stain TEM image of plain 6HB structures. c, Negative-stain TEM image of native CCMV particles. d, EMSA shows electrophoretic mobility decrease of 6HB upon complexation with CPs when increasing ε. e, Development of a single layer of CPs on the 6HB origami template using ε ≤ 2k. f, Subsequent development of a second CP layer on top of e. g, Observed size distributions (in diameter) for plain 6HB (blue) and 6HB complexed at ε = 2k (grey) or at ε = 10k (green). The image width of all TEM images corresponds to 500 nm.
