Fig. 6: Multiplexed detection of miRNAs in human serum.
a, Mean percentage delay of barcoded probes (not demultiplexed). Serum, after centrifugation with 10 kDa molecular weight cut-off spin filter, was incubated with 40 barcoded probes (30 nM) and compared to zero serum control (one-tailed t-test P > 0.05, n = 3, ntotal events = 194,115). b, Heat map of P values comparing percentage delayed events ±serum (one-tailed t-test, n = 3, ntotal events = 194,115). c, Significantly upregulated miRNAs per patient (one-tailed t-test *P ≤ 0.05, **P ≤ 0.01, n = 3, ntotal events = 194,115). Exact P values are available from the source data file for this figure. Data are presented as mean ± s.d. All experiments were performed in sequencing buffer.
