Extended Data Fig. 1: Characterization of insulin receptors at the adipocyte cell membrane.
From: Multivalent insulin receptor activation using insulin–DNA origami nanostructures
a, Histogram of the number of localizations per cluster. Red line represents the Gaussian fit showing multiple peaks representing one, two, three, etc., docking sites. Peaks at 7 ± 0.14 and 13 ± 0.61 both correspond to signal from a single receptor since each primary antibody can be bound by one or two nanobodies conjugated to one DNA-PAINT docking site. The table shows the range of localizations that define the number of receptors. b, Insulin receptor cluster outlines in adipocytes treated with 10 nM insulin for 10 mins and corresponding control. Image representative of 3 independent experiments. Scale bar, 2 µm. c, Table showing the number of IR and IR clusters per 10 µm2 region (mean ± SD), number of receptors per cluster (median and interquartile range), and cluster diameter (mean ± SD) plotted in Fig. 1c, in control and insulin-treated adipocyte cells. d, Characterization of the distance between the edges of neighboring IR clusters in Control (median=178.9 nm, 95% CI = 176.1-181.4 nm) and insulin treated (median=175.7 nm, 95% CI = 173.2-178.3 nm) adipocytes. Boxplot data: center line defines median, whiskers indicate 5-95 percentile. n = 16897 (control) and n = 16374 (10 nM Insulin) pairs of clusters examined over 10 cells for each condition. Data points outside whisker limits are omitted from plot.
