Extended Data Fig. 5: Quantification of the NI in different experimental conditions.
From: Dynamin A as a one-component division machinery for synthetic cells
(a) Quantification of the NI of a large dataset of necks from chains of dumbbells. This plot shows the data pooled from the three experimental conditions shown in Fig. 2a,b and Supplementary Fig. 6b (n = 190 chains of dumbbells), and are decomposed into three peaks using a Gaussian Mixture Model. The three peaks correspond to open necks, hemi-scission and full scission. For each data point, the fluorescent lipids of one lobe were bleached and their recovery was followed over time until reaching the plateau. The final intensity was used for calculating and plotting the NI. (b) Plot showing the NI of dumbbells having only bare membrane (left), and pie-chart (right) indicating the fraction of open necks, hemi-scission and full scission events (BN; 63 necks from 5 independent preparations).
