Extended Data Fig. 6: Membrane remodelling by DynA in different experimental conditions.
From: Dynamin A as a one-component division machinery for synthetic cells
(a) pie-charts indicating the fraction of open necks, hemi-scission and full scission events in the presence or absence of GTP. Both samples include cholesterol-oligo, CN and DynA. With GTP, n = 59; without GTP n = 10. (b): lack of binding of DynA-D2 domain to membrane containing 7.5% DOPG in the absence of Mg++. Scale bar: 5µm. (c) DynA assembly at the neck results in neck constriction. The plot shows the neck diameter in the presence or absence of DynA, estimated based on dye recovery upon photobleaching. In open necks and in the absence of DynA, the average inner diameter of necks was 134 ± 108 nm (mean ± SD). In the presence of DynA, the width of open necks appeared more constricted, with an average diameter of 57 ± 33 nm (mean ± SD). n = 8 without DynA; n = 9 with DynA. The estimation has been performed according to the procedure detailed in Ref. 18.
