Extended Data Fig. 9: Lobe detachment upon scission and visualization of nanotube.
From: Dynamin A as a one-component division machinery for synthetic cells
(a) confocal image of liposomes that underwent full scission (identical to Fig. 4e). (b) Same image as in (a) but with increased gain, causing overexposure of the liposome membrane. This condition confirms that the two lobes indeed underwent scission, since no nanotube is visibly connecting the two liposomes. Note that in panel d, a nanotube was visible upon overexposure. Scale bar: 10µm. (c) Confocal image of two liposomes under standard imaging conditions which allow to clearly see the liposome membrane without overexposure. Upon bleaching of one liposome (marked with a blue asterisk) full lipid recovery could be observed. (d) Same images as in panel c with increased gain, causing overexposure of the liposome membrane. Such conditions reveal that a membrane nanotube is connecting the two liposomes and allows full lipid recovery. Scale bar: 5µm.
