Extended Data Fig. 3: Recovery times in FRAP experiments and example of open neck in the absence of DynA. | Nature Nanotechnology

Extended Data Fig. 3: Recovery times in FRAP experiments and example of open neck in the absence of DynA.

From: Dynamin A as a one-component division machinery for synthetic cells

Extended Data Fig. 3: Recovery times in FRAP experiments and example of open neck in the absence of DynA.

(a) Median of the duration of recovery times in FRAP experiments. Acquisition was stopped when no further lipid recovery was apparent. This quantitation is obtained from datasets such as shown in Fig. 2a,b and Supplementary Fig. 5b. n = 190 necks from 24 independent experiments. Data are presented as median values +/− SEM. (b) example of FRAP experiment showing full lipid recovery (open neck) on a chain of dumbbells without reconstitution of DynA. The orange asterisk indicates the control lobe, the blue asterisk indicates the bleached lobe. Scale bar: 5µm.

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