Extended Data Fig. 4: Lobe detachment upon scission.
From: Dynamin A as a one-component division machinery for synthetic cells
(a) Confocal images of a dumbbell with a DynA cluster (in green) localized at neck. The dumbbell was imaged just as scission was occurring and the two lobes are drifting apart. (b) same dumbbell as in panel a. After lobes separation, FRAP analysis confirmed that the membrane connection between the two lobes had been lost and that no lipid recovery occurred. The bleached lobe is indicated by a blue asterisk, and its position is revealed by the low fluorescent signal of DynA binding to the membrane in the overexposed image.
