Extended Data Fig. 9: Influence of F-no-hinge and D–F-ss-hinge on angle distribution in D–F.
From: A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower
The example of a TEM image for the F-no-hinge structure (a) shows how large the angular range is when the double-stranded structure is absent in the flexure region making it impossible to obtain the correct structure that determines the angular shape of the origami structure. The F-no-hinge (b) constructs shows a wide and flat distribution (c,d; F-ss-hinge: olive, n = 1682, 28 micrographs, 107.35° ± 41.03°, min=0.95° max=179.87° median = 112.78°) with obtuse angle. When combining the F-no-hinge origami with the D unit the angle is reduced to 71° ± 17° with a narrower distribution (D–F-ss-hinge No Transcription: wine, n = 398, 21 micrographs, 70.71° ± 17.12°, min=24.52° max=125.08° median = 70.83°). Under transcription conditions, the average distribution angle shifts to 55° ± 20° and becomes slightly larger than expected for the transcription sample (D–F-ss-hinge Transcription: green, n = 462, 84 micrographs, 54.81° ± 19.96° mean and SD, min=9.21° max=117.00° median = 55.63°). d) Box-plots show how the distribution for the F-no-hinge (olive) is widely spread over a wide range of angles, having a difference between Q3 and Q1 of 54° while in the D–F this range is reduced to 23° in absence of transcription (wine) and 29° in presence of transcription (green). ***1: p = 4 × 10−138, ***2: p = 6 × 10−229, ***3: p = 2 × 10−33. p-values obtained with two-tailed, heteroscedastic t-test. Box-plot edges: 25th and 75th percentiles. Box lines: 50th percentile. Whisker size: 1.5× the IQR, grey dots: outliers. Error ranges: mean and S.D.
