Extended Data Fig. 2: Assembly verification of the NE by AFM and TEM.
From: A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower
Assembly verification of the nanoengine by AFM and TEM. (a) Detailed AFM image of the origami structure; green line: spans the streptavidin molecules attached to the respective origami arm; red line: marks the cross-section of the opposing origami arm. (b, c) Height profiles of the green and red lines confirming the distance of the streptavidin molecules on the respective origami arm to be spaced exactly 21 nm as designed (b), and the cross-section of the opposing origami arm to be exactly 9 nm as designed (c). (d) Comparison between the nanoengine design (left) and a 2D average image obtained from negative stained TEM micrographs (right). The 2D average shows an origami arm length of 60 nm as per design. The presence of streptavidin, depicted as green dots on the image on the left are visible in the 2D average as 21-22 nm evenly spaced bright dots on the outside of the horizontal origami arm. The pink arrows point to dsDNA-t, in particular where the strand contacts the origami, that is visible as a fuzzy shadow in the 2D average image. The blue arrow points to a nicked corner of the upper origami arm, a feature found in the 2D average.
