Fig. 3: Ex vivo experiment using WT and rd1 mouse retina.
a, Schematic of the experimental setup for an ex vivo experiment using WT and rd1 mouse retina. b, Optical stereomicrograph of a device consisting of 36 stimulating and recording 3D LM microelectrode pairs (pitch, 40 μm). Scale bar, 400 μm. c, VEP and EEP of WT and rd1 mouse retina. Data are mean ± s.d. with n = 5 biologically independent mice. Significance was calculated using an unpaired one-tailed t-test: P = 2.860441 (n.s.), P = 0.0000234 (****, left), P = 0.000017 (****, right). d, EEPs of WT and rd1 mouse retina (pulse width, 1 ms; current, 2 μA) in the dark state. The red dashed line indicates the initiation of stimulation. e, EEPs of WT mouse retina under light (470 nm) exposure with different intensities during the operation of the artificial retina with flat-surface-type stimulation electrodes. f, EEPs of rd1 mouse retina under light (470 nm) exposure with different intensities during the operation of the artificial retina with flat-surface-type stimulation electrodes. g, Firing rates of EEPs as a function of illuminated light intensities for WT and rd1 mouse retina. Data are mean ± s.d. with n = 5 biologically independent mice. h, EEPs of WT mouse retina under light (470 nm) exposure during the operation of artificial retina with different heights of the 3D LM microelectrode. i, EEPs of rd1 mouse retina under light (470 nm) exposure during the operation of the artificial retina with different heights of the 3D LM microelectrode. j, Firing rates of evoked RGC spikes as a function of heights of 3D LM microelectrodes for WT and rd1 mouse retina. Data are mean ± s.d. with n = 5 biologically independent mice.
