Extended Data Fig. 1: NELISA detection of SCCA based on FGpTD8 and alkaline phosphatase.
From: Nanopore-based enzyme-linked immunosorbent assay for cancer biomarker detection
(a) The enzymatic cleavage of FGpTD8 by alkaline phosphatase and corresponding translocation current signal changes. (b) Quantification of SCCA by NELISA using FGpTD8. Linear equation of the standard working curve: y = -25.26 lgx + 343.31; R2 = 0.996; LOD is 100 pg/mL. (c) Specificity of alkaline phosphatase and FGpTD8 for the detection of SCCA. Different types of protein antigens (1.0 μg/mL SCCA, 1.0 kU/mL CA125, 1.0 μg/mL NSE, 600.0 ng/mL AFP, 1.0 kU/mL CA19-9 and 500.0 ng/mL CEA) are used in the test. All data were acquired in the buffer of 3.6 M KCl, 10.0 mM PBS, pH 5.0 in trans, 1.0 M KCl, 10.0 mM PBS, pH 5.0 in cis, with the transmembrane potential held at +200 mV. Number of individual experiments n = 3. Each data comes from three independently prepared standard solution samples of the same concentration and three independent nanopore translocation experiments. Data are presented as mean ± SD.
