Extended Data Fig. 2: NELISA detection of NSE based on PBAP-FGED8 and glucose oxidase.
From: Nanopore-based enzyme-linked immunosorbent assay for cancer biomarker detection
(a) The enzymatic cleavage interaction between PBAP-FGED8 and H2O2 derived from glucose oxidase and corresponding translocation current signal changes. (b) Quantification of NSE by NELISA using PBAP-FGED8. Linear equation of the standard working curve: y = 39.55 lgx – 29.73; R2 = 0.995; LOD is 100 pg/mL. (c) Specificity of glucose oxidase and PBAP-FGED8 for the detection of NSE. Different types of protein antigens (1.0 μg/mL NSE, 1.0 μg/mL SCCA, 1.0 kU/mL CA125, 600.0 ng/mL AFP, 500.0 ng/mL CEA and 1.0 kU/mL CA19-9) are used in the test. All data were acquired in the buffer of 3.6 M KCl, 10.0 mM PBS, pH 5.0 in trans, 1.0 M KCl, 10.0 mM PBS, pH 5.0 in cis, with the transmembrane potential held at +200 mV. Number of individual experiments n = 3. Each data comes from three independently prepared standard solution samples of the same concentration and three independent nanopore translocation experiments. Data are presented as mean ± SD.
