Extended Data Fig. 1: DV1@O-Colase nanogels potentiated the anti-tumor therapy of αPD-1.
From: A collagenase nanogel backpack improves CAR-T cell therapy outcomes in pancreatic cancer
a, Schematic diagram for the experimental protocols of anti-tumor evaluation of DV1@O-Colase nanogels in combination with anti-PD-1 therapy. b, Representative bioluminescence images and c, quantification of bioluminescence intensity of tumor growth in C57BL/6 mice during therapy (n = 5 biologically independent mice). d, The body weight of mice during therapy (n = 5 biologically independent mice). e, Representative FCM scatter blots and quantification of the frequency of CD8+ T cells in tumors (n = 5 biologically independent mice). f, Representative FCM scatter blots and quantification of the expression of granzyme B in tumor infiltrating CD8+ T cells (n = 5 biologically independent mice). g, Representative FCM scatter blots and quantification of the expression of IFN-γ in tumor infiltrating CD4+ T cells (n = 5 biologically independent mice). h, Representative FCM scatter blots and quantification of the frequency of Tregs in tumors (n = 5 biologically independent mice). i, Representative FCM scatter blots and quantification of the frequency of MDSCs in tumors (n = 5 biologically independent mice). Data in e–i were recorded in another parallel experiment of b. The experiments were repeated twice independently, with similar results (b–i). Data in c–i were presented as mean ± SD. P values were determined by two-way ANOVA test (c) or one-way ANOVA test (e–i). **P < 0.01, ****P < 0.0001.
