Fig. 4: Lumen charge-dependent gating behaviour measured with different aerolysin, α-HL and MspA mutants.
From: Lumen charge governs gated ion transport in β-barrel nanopores
a–c, Gating behaviour measured with different aerolysin (a), α-HL (b) and MspA (c) mutants, showcasing the great tunability of gating by changing lumen charge. Mutants gating at negative potentials shown in green, at positive potentials in orange, mutants showing low levels of gating in grey and mutants that gate at both polarities in purple. Each plot shows the open-pore IV curve in black, showing the differences in rectification. Further, each plot shows ±1 standard deviation of the mean over the number of experiments n plotted as a grey area. All experiments were conducted in 1 M KCl buffered to pH 6.2 with 10 mM phosphate. Sinusoidal forcing was applied at 0.1 Hz and 200 mV amplitude (100 mV for MspA wt and m2MspA).
