Extended Data Fig. 3: Model of ionic gating in aerolysin.
From: Lumen charge governs gated ion transport in β-barrel nanopores
a. Schematic representation of depletion interactions. In the open state, the pore is relatively wide and permeable to ions. In the closed state, ions face an energy barrier owing to the smaller radius, resulting in depletion interactions. b. Simplified energy profile for the ionic energy barrier μex. c. Energy landscape of the pore under zero voltage, owing to depletion interactions (orange curve, concentration 1 M; blue curve, concentration 1.2 M). d. Parametrization of the gating model e. Schematic representation of the model’s derivation. f-k. Results of the model, showing the number of counterions neutralizing the fixed lumen charge (f), the free energy of the closed state (g) and the closed state probability for various lumen charge positions (h, j), voltage frequency (i) and temperature (k). All parameters are detailed in the text.
