Fig. 1: Schematic of the PLASTIQ workflow for assessing DNA origami integrity using 1 µl of blood. | Nature Nanotechnology

Fig. 1: Schematic of the PLASTIQ workflow for assessing DNA origami integrity using 1 µl of blood.

From: Resolving DNA origami structural integrity and pharmacokinetics in vivo

Fig. 1: Schematic of the PLASTIQ workflow for assessing DNA origami integrity using 1 µl of blood.The alternative text for this image may have been generated using AI.

The nanostructure of interest is designed to contain a set of LSPs, consisting of two contiguous staples that can be ligated due to the presence of a 5′-phosphate group (represented as the circled P) on one of them. Ligation of the LSPs occurs only when they are held in proximity by the scaffold in an intact origami structure. This is followed by amplification for quantification, thereby assessing the structural integrity of the DNA origami.

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