Fig. 2: In vitro proof of concept to assess DNA origami integrity with PLASTIQ.
From: Resolving DNA origami structural integrity and pharmacokinetics in vivo
a, PLASTIQ workflow in vitro. LSPs consisting of two contiguous staples with the 5′-end breakpoint containing a phosphate group for ligation and flanked by a protruding primer targeting region (pink and blue) for pooled PCR. After ligation and PCR, the products are resolved by PAGE. b, Representative cyro-electron microscopy image of the Wrod origami (left). Scale bar, 100 nm. TEM micrographs of the Lrod (right). Scale bar, 50 nm. c, Location of the LSPs in Wrod. d, Products of ligated and amplified LSPs on PAGE gel from the Wrod origami containing either all nine LSPs pairs (all) or only one LSP (numbered 1–9) to resolve the bands from the pooled PCR with all LSPs. Data are representative of three independent experiments with similar results. e, PAGE gel visualization of PCR amplification with primers targeting the scaffold or an LSP from either non-denatured (ND) or denatured (D) Wrod sample. Data are representative of three independent experiments with similar results. f, Location of the LSPs in the Lrod. g, Products of ligated and amplified LSPs on PAGE gel from the Lrod origami containing either all eight LSPs pairs (all) or only one LSP (numbered 1–8) to resolve the bands from the pooled PCR with all LSPs. h, PAGE gel visualization of PCR amplification with primers targeting the scaffold or LSPs from either ND or D Lrod.
