Fig. 4: In vivo quantification of DNA origami integrity using PLASTIQ followed by qPCR.
From: Resolving DNA origami structural integrity and pharmacokinetics in vivo
a, PLASTIQ workflow applied to blood samples, followed by qPCR analysis. b,c, Time-dependent changes in origami integrity following i.v. (b) or i.p. (c) injection across multiple mice. n = 3. d–g, Representative graphs showing the quantification of individual LSPs for one individual mouse resulting in the averages in b and c. The blood concentrations of each ligated LSP were determined by qPCR following i.v. (d and f) or i.p. (e and g) injection of bare Wrod (d and e) and PEGylated (f and g) PEG-Wrod. The overall origami integrity curve is generated by averaging the signals from all ligated LSPs.
