Fig. 5: Pharmacokinetic modelling of DNA origami in vivo.
From: Resolving DNA origami structural integrity and pharmacokinetics in vivo
a, i.v. injection model based on linear elimination kinetics. b,c, Fitted ligated LSP blood concentration profiles plotted on top of i.v. injection experimental data for Wrod (b) and PEG-Wrod (c). The plots for each ligated LSP shown separately are provided in Supplementary Fig. 10. d, Illustration of i.p. injection model based on two compartment kinetics in which an initial peritoneal absorption phase is followed by an elimination phase in blood. e,f, Fitted ligated LSP blood concentration profiles plotted on top of i.p. experimental data for Wrod (e) and PEG-Wrod (f). The plots for each ligated LSP shown separately are provided in Supplementary Fig. 11. Smooth model curves for observable blood concentration and hidden inferred peritoneal concentration for i.p. injection samples are shown in Supplementary Fig. 12. g, Artificially generated kinetic profile for a repeated dose with 30-min interval, plotted using mean fitted parameter values for i.p. injection of PEG-Wrod. Artificially generated kinetic profiles with 60- and 120-min intervals are provided in Supplementary Fig. 13. h, Swarm plots of parameter values (absorption and elimination rate constants, as well as the initial concentration Qo) fitted according to their respective models (two compartment or single compartment) using nonlinear least squares optimization for the i.v., i.p., PEG-Wrod and Wrod conditions, with each point representing an independently fitted LSP. The spread, thus, captures the variability between individual LSPs. The statistical results are shown in Supplementary Table 1.
