Extended Data Fig. 1: Characterization and in vitro application of the PLASTIQ method to UV-crosslinked lattice-based origami UV-Lrod.
From: Resolving DNA origami structural integrity and pharmacokinetics in vivo
a, Agarose gel electrophoresis of magnesium screening for folding of the UV-Lrod. L: 1 kb DNA ladder, S: p7560 scaffold, 0-20: increasing concentrations of MgCl2 in mM from 0 mM to 20 mM. Data are representative of three independent experiments with similar results. b, Location of the LSPs in the UV-Lrod. Data are representative of three independent experiments with similar results. c, Transmission electron microscopy micrographs showing the UV-Lrod before (non-irradiated) and after (irradiated) UV irradiation. Scale bars 50 nm. Data are representative of two independent experiments with similar results. d, Illustration of the UV-Lrod showing the location of all LSPs (left). On the right, a PAGE gel showing the products the non-irradiated (NI) or irradiated UV-Lrod (I) origami after ligation and pooled PCR amplification with primers targeting the eight LSPs (LSP) or the scaffold (S). In comparison, the products of non-ligated origami after pooled PCR amplification. L: DNA ladder, NI: non-irradiated UV-Lrod, I: irradiated UV-Lrod, LSP: pooled PCR amplification targeting the LSPs, S: pooled PCR amplification targeting the scaffold. Data are representative of three independent experiments with similar results.
