Fig. 1: Glycan atlassing reveals the nanoscale organization of the glycocalyx.
From: Glycan atlassing enables functional tracing of cell state
a, Schematic of the glycocalyx. b, Labelling. Schematic overview of (i) lectin labelling and (ii) metabolic labelling, targeting sialic acids. c, Principle of NN analysis. (i) The distance between every target location and its NN in the same and in all other channels are calculated. (ii) NN histograms of one representative channel. The colour-coded matrix reports on all inter- and intra-channel peaks of the NN distance histograms. (iii) PCA is conducted on the NN distance matrices. d, Principle of GlyCo. Two or more target locations from different channels are grouped if their distance is smaller than a threshold. (i) Five example classes, each containing two target locations, are shown. (ii) The most frequent classes found in a sample are collected. (iii) PCA is conducted on the NN distance matrices and the histograms from GlyCo.
