Fig. 5: Glycan atlassing traces immune cell activation.
From: Glycan atlassing enables functional tracing of cell state
a, (i) Individual channels in greyscale. (ii) Merged data of individual channels (the white arrowhead indicates the cell analysed here; Fig. 2 shows the colour map). The inset with the small arrowhead denotes the area represented in b. (iii) FoV in bright field. b, Representative example of clustering localization clouds to single target locations. (i) Raw localizations. (ii) Clustered localizations. c, NN histograms of one representative channel (relationship of PHA-L to all channels). d, Maxima from NN histograms across all comparisons. e, Lectin class distribution from GlyCo. The ten most frequent classes and their spatial densities are shown. f, Spatial arrangement of the classes shown in e. g–i, PCA plots for (i) GlyCo and (ii) NN peak distances for all the samples investigated, comparing the characteristic glycosylation patterns between activated and non-activated NK (g), CD4+ T cells (h) and neutrophils (i). n = 4 independent isolations, two cells per staining for NK cells, n = 1 isolation, two independent seedings and stainings, two cells per staining for CD4+ T cells; n = 2 isolations, two independent seedings and stainings, one cell per staining for neutrophils. Scale bars, 10 µm (a); 100 nm (b).
