Extended Data Fig. 4: Image size comparison of CHIDO, the vortex DSF, raPol standard DSF and raMVR. | Nature Photonics

Extended Data Fig. 4: Image size comparison of CHIDO, the vortex DSF, raPol standard DSF and raMVR.

From: Six-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope

Extended Data Fig. 4

(a-d) Images and (e) line profiles of (a) CHIDO (x -polarized channel), (b) the Vortex DSF, (c) raPol standard DSF (radially polarized channel), and (d) raMVR (azimuthally polarized channel) for isotropic emitters located at z = 250 nm, z = 750 nm (best focus), and z = 1250 nm. The nominal focal plane zf = 1200 nm. Colourbar: normalized intensity; scale bar: 500 nm. (e) Line profiles of the DSFs in (a-d). Yellow: CHIDO; green: Vortex DSF; gray: raPol standard DSF; red: raMVR.

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