Extended Data Fig. 1: Tissue phantom study of multispectral imaging in 850-1000 nm.
a, Spectral phasor transformation for a representative multispectral stack (left panel). Pixel intensities across excitation wavelengths are transformed into spectra (middle panel) and represented as points on a four-quadrant polar plot (right panel). Plot coordinates denote the real (G) and imaginary (S) components of the Fourier transform. Phasor point positions indicate spectral profiles: counterclockwise movement from (-1,0) represents spectral red shift, while distance from the center reflects spectral bandwidth. b, Capillary imaging setup for the tissue phantom study in the 850-1000 nm range. Component 6, 7 and 9: EP737, EP765, and EP772. c. Phasor plots of datasets across varying depths. d, Normalized excitation spectral signatures. Solid lines represent mean values averaged across depths; shaded areas indicate standard deviation (s.d.). e, Normalized RMSE of excitation spectral signatures at depths of 1-3 mm, compared to the reference depth of 0 mm. Each point represents one fluorophore. 1500-1700 nm, n = 9; 850-1000 nm, n = 3. Data are presented as mean values ± s.d. f, Unmixed capillary signals and cross-sectional profiles (bottom) at representative phantom depths of 0 and 3 mm. g, h, Contrast (g) and bleed-through (h) values for each unmixed channel at varying phantom depths. Each point represents one fluorophore. n = 3 for 850-1000 nm. Data are presented as mean values ± s.e.m.
