Extended Data Fig. 3: Biodistribution study of EP fluorophores with different formulations and delivery routes.

a, c, e, Schematics illustrating in vivo biodistribution tracking of: EP772/F127 micelles (a) in a mouse model bearing an in situ CT26 tumor via intravenous injection; EP772/BSA complex (c) via intravenous injection in two normal mice. Images were obtained from two mice to minimize the impact of multiple laparotomies; EP772/F127 micelles (e) within the normal mouse intestinal system via intragastric administration. b, Representative in vivo fluorescence images of EP772/F127 micelles at different time points. Vascular signals diminish by 72 h post-injection, while tumor signals remain detectable. Scale bar, 5 mm. d, Representative in vivo fluorescence images showing EP772/BSA accumulation in mesenteric lymph nodes at different time points post-injection. Fluorescence in mesenteric lymph nodes is detectable at 1 h post-injection and persists up to 72 h. Scale bar, 5 mm. f, Representative in vivo fluorescence images of the mouse intestinal system at different time points post-administration. Fluorescent signals are first detected in the small intestine at 0.5 h post-administration. By 2.5 h, signals in the small intestine diminish, and fluorescence becomes predominantly localized to the cecum at later time points. Scale bar, 10 mm.