Extended Data Fig. 6: Cell size and morphology is minimally affected by blue light activation.

a, Nuclear structure and morphology in both FUS Corelet cells expressing H2B-miRFP and in a control line expressing only H2B-miRFP is qualitatively unaffected by typical imaging protocols. b, Nuclear size is identical before and after 20 minutes of activation, based on segmentation on pre-activated ferritin “core” (GFP) image and post-activation H2B-miRFP image, suggesting no major change in osmotic environment of the nucleus. c. Diffusive exponent α is unaffected by volume fraction, remaining at approximately 0.5 for volume fractions ranging from 5% to 20%, suggesting that the same material properties of chromatin are measured by droplet MSDs regardless of volume fraction. d, Effective diffusion coefficient decreases with droplet volume fraction, consistent with data in Fig. 6 suggesting that droplet size is inversely correlated with diffusion coefficient, since cells with higher volume fractions have larger average droplet sizes.