Fig. 2: Mutant analysis reveals that the C terminus of FoxA1 drives DNA condensation.
DNA envelope width measurements for FoxA1 mutants. The data are binned every 2 μm and the mean ± s.d. (for both the envelope width and the strand length) are shown in black for each mutant and in blue for the control (n = 45). a, Sequence-specific DNA-binding mutant NH-FoxA1 condenses DNA (n = 30). DBD, DNA-binding domain. b, Non-sequence-specific DNA-binding mutant RR-FoxA1 condenses DNA (n = 28). c, N-terminal truncation of FoxA1, ΔN-FoxA1, condenses DNA (n = 13). d, C-terminal truncation of FoxA1, ΔC-FoxA1, inhibits DNA condensation (n = 44). In all conditions, the protein concentration was 10 nM. See Supplementary Fig. 1 for representative protein–DNA images of the FoxA1 mutants.
