Extended Data Fig. 1: Effect of passaging on the deformability of primary breast tumour and normal epithelial cells.

Left) Distribution of the maximum relative cell deformations (observed at t = 3 s) for a breast cancer sample stretched at the end of passage number 1-3 (sample size: P1 = 164 cells, P 2 = 496 cells, P3 = 309 cells). Right) Relative deformation distribution epithelial cells from breast reduction (HMEpC) stretched at the end of passage number 2 and 10 (sample size: P2 = 352 cells, P10 = 702 cells). With increasing passage number more and more deformable cells are found. Time between passaging was 7 to 10 days.