Table 2 Culturomic conditions for the isolation of bacteria from either urine and kidney stones13 or stool54

From: Standardization of microbiome studies for urolithiasis: an international consensus agreement

Media

Temperature

Atmosphere

Pre-incubation

Incubation period

Urine/kidney stone

Blood agar

37 °C

Aerobic

No

48 h

Blood agar + colistin and nalidixic acid

37 °C

Anaerobic

No

48 h

CDC anaerobe blood agar

37 °C

5% CO2

No

48 h

Chocolate agar

37 °C

5% CO2

No

48 h

Stool

Blood agar

37 °C

Aerobic

Yes

Up to 30 days

Blood agar + rumen fluid

37 °C

Anaerobic

Yes

Up to 30 days

Blood agar + marine broth

37 °C

Microaerophilic

Yes

Up to 30 days

Blood agar + trypticase soy broth

37 °C

Microaerophilic

Yes

Up to 30 days