Table 2 Culturomic conditions for the isolation of bacteria from either urine and kidney stones13 or stool54
From: Standardization of microbiome studies for urolithiasis: an international consensus agreement
Media | Temperature | Atmosphere | Pre-incubation | Incubation period |
|---|---|---|---|---|
Urine/kidney stone | ||||
Blood agar | 37 °C | Aerobic | No | 48 h |
Blood agar + colistin and nalidixic acid | 37 °C | Anaerobic | No | 48 h |
CDC anaerobe blood agar | 37 °C | 5% CO2 | No | 48 h |
Chocolate agar | 37 °C | 5% CO2 | No | 48 h |
Stool | ||||
Blood agar | 37 °C | Aerobic | Yes | Up to 30 days |
Blood agar + rumen fluid | 37 °C | Anaerobic | Yes | Up to 30 days |
Blood agar + marine broth | 37 °C | Microaerophilic | Yes | Up to 30 days |
Blood agar + trypticase soy broth | 37 °C | Microaerophilic | Yes | Up to 30 days |