Extended Data Fig. 1: Effects of synthetic CLE peptide application on NCED3 expression and stomatal closure in leaves, and movement of CLE25 peptide from roots to leaves.
From: A small peptide modulates stomatal control via abscisic acid in long-distance signalling
a, NCED3 expression after application of 5 μM of each synthetic CLE peptide to roots for 3 h, or in response to dehydration stress for 3 h in leaves of wild-type plants (n = 4 biological replicates). **P < 0.01, no significant difference (NS) among treatment conditions as analysed by two-tailed Student’s t-test (see Methods for exact P values). b, NCED3 expression in leaves of wild-type plants after application of 1 μM peptide to roots for 3 h (n = 6 biological replicates). c, ABA content in leaves after application of 1 μM peptide to roots for 3 h (n = 8 biological replicates). d, Comparison of peptide sequences of CLE25 and CLE26. e, Typical images of wild-type (n = 6 biological replicates), 1 μM-ABA- or 1 μM-CLE25-induced stomatal closure (n = 4 and 6 biological replicates, respectively). Scale bars, 20 μm. f, Roots of whole plants were incubated without (0 h on x axis, n = 547) or with 0.01% acetonitrile (mock, n = 519), ABA (n = 562) or each CLE peptide (n = 546, CLE26; n = 578, CLV3; n = 762, CLE46; n = 561, TDIF) for 3 h. Data are from three experiments. **P < 0.01, ***P < 0.001 as analysed by one-way ANOVA followed by a Tukey’s (b, c) or a Tukey–Kramer (f) post hoc test. g, h, Detection of non-labelled (g) and isotope-labelled (h) CLE25 peptide by nLC–MS/MS. These experiments were repeated two times independently with similar results. Hyp, hydroxyproline.
