Extended Data Fig. 7: Immunohistochemistry of epithelial markers within blastoids.
From: Blastocyst-like structures generated solely from stem cells
a, Cross-section of a blastoid stained with an antibody against E-cadherin. Maximum projection of 5 images taken with a 1-µm step. b, Phalloidin staining of the cytoskeletal molecule F-actin and Hoechst staining of DNA show the localization of actin at cell–cell junctions. Maximum projection of 40 images taken with a 1-µm step. c, Antibody against the tight junction molecule ZO-1. Section of 0.5 µm (top). Maximum projection of 4 images taken with a 0.5-µm step (bottom). d, Antibody against the apical molecule PKCz and Hoechst staining of DNA. Section of 1 µm (top); close up (bottom). All pictures were taken with a PerkinElmer Ultraview VoX spinning disk microscope combined with a Leica SP8. Images are representative of three experiments.
