Extended Data Fig. 1: Additional details of the nanoscale synthesis and affinity ranking workflow.
a, Reaction solutions are dosed using a TTP mosquito liquid-handling robot that handles increments of 20 nl. b, c, UPLC–MS analysis confirms the presence of products from crude reactions (b), which is visualized in heat maps (c). d, For the ASMS assay, productive reactions are mass-encoded, pooled and incubated with a protein of interest. e, f, Elution through a size-exclusion column separates protein-bound compounds from unbound compounds (e), and binders can be observed by high-performance liquid chromatography–mass spectrometry (HPLC–MS) following denaturation of the protein–ligand complex (f). g, Decreasing the concentration of protein in the ASMS assay increases the competition for binding so that ligands can be categorized as high, medium or low affinity. h, Scale-up of compounds and purification by using a traditional approach. i, Measurement of IC50 in a functional assay enables comparison of the new method to existing assay technology. See Supplementary Information for additional details.
