Extended Data Fig. 2: Cryo-EM analysis of double- and single-ring GSDMA3 pores. | Nature

Extended Data Fig. 2: Cryo-EM analysis of double- and single-ring GSDMA3 pores.

From: Cryo-EM structure of the gasdermin A3 membrane pore

Extended Data Fig. 2: Cryo-EM analysis of double- and single-ring GSDMA3 pores.

a–c, Gold-standard FSC plots from two half-reconstructions refined separately in RELION for the 27-fold double-ring pore map (a), the 27-fold single-ring pore map (b) and the 28-fold single-ring pore map (c). Model-to-map correlations are shown for the 27-fold single-ring pore (b). d, e, Local resolution estimation generated by ResMap on the two half maps separately refined in a gold-standard procedure in RELION. Local resolutions are colour-coded on the densities. Highest resolution is observed at the β-barrel domain for both the single-ring pore (d) and the membrane-inserted ring of the double-ring pore (e). The globular domains and the additional ring exhibit relatively low resolution.

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