Extended Data Fig. 5: Proliferation in HpBDs and reactive ductules.
From: De novo formation of the biliary system by TGFβ-mediated hepatocyte transdifferentiation
a, Size distribution of wsCK-positive DBA-positive hilar cholangiocyte clones in P90 Alb-cre+/−Rbpjf/fHnf6f/fR26R-Confetti+/− (n = 2) and Alb-cre+/−R26R-Confetti+/− (n = 2) mouse livers. **P = 0.0079 for three cells and ***P = 0.00092 for seven cells, two-sided Student’s t-test. b, Immunofluorescence of reactive ductules in hepatocyte-fate-traced P32 Alb-cre+/−Rbpjf/fHnf6f/fR26ZG+/+ mouse liver (n = 3). c, Size distribution of wsCK-positive DBA-negative peripheral cholangiocyte clones in P90 Alb-cre+/−Rbpjf/fHnf6f/fR26R-Confetti+/− (n = 2) and Alb-cre+/−R26R-Confetti+/− (n = 2) mouse livers. *P = 0.032 for one cell, *P = 0.024 for two cells, *P = 0.020 for three cells and *P = 0.014 for four cells, two-sided Student’s t-test. Horizontal lines in a and c denote mean values. d, Immunofluorescence and breakdown of OPN-positive KI67-positive cells based on CK19 expression in P54 Alb-cre+/−Rbpjf/fHnf6f/f mouse liver (n = 4). Arrowheads indicate OPN-positive KI67-positive CK19-negative cells. e, Immunofluorescence of liver of >P120 Alb-cre+/−Rbpjf/fHnf6f/f and Rbpjf/fHnf6f/f mice after DDC diet feeding for 2 (n = 1 each), 4 (n = 3 each) and 6 (n = 1 each) weeks. f, Immunofluorescence of liver and breakdown of OPN-positive cells based on hepatocyte fate tracing in >P120 Alb-cre+/−Rbpjf/fHnf6f/fR26ZG+/+ (n = 4) and Rbpjf/fHnf6f/fR26ZG+/+ (n = 3) mice fed DDC diet for 5 weeks starting 1 week after hepatocyte fate tracing was induced. Data in d and f are mean ± s.e.m. Scale bars, 100 µm (b, e, f), 50 µm (d).
