Extended Data Fig. 8: Characterization of two-cell distal open chromatin in human embryos.

a, Heat maps showing the ATAC-seq signals and CpG density for promoter and distal ATAC-seq peaks at the two-cell stage. b, Top, Venn diagram showing the overlap between two- and eight-cell distal ATAC-seq peaks. Bottom, motifs identified in two- and eight-cell embryo shared distal peaks as well as peaks specific for each stage are also shown. c, The enrichment of human ES cell H3K4me1 and H3K27ac marks⁴⁷ around two-cell-specific distal peaks or two-to-eight-cell shared distal peaks is shown. A random set of peaks that match the lengths of individual two-cell-specific ATAC-seq peaks was used as a control. The upstream and downstream regions are 2 × peak lengths away from peak boundary. d, Bar chart showing the percentages of ATAC-seq peaks that overlap with oocyte PMDs for total peaks, peaks shared by various stages, or peaks specific for each stage. e, Heat maps showing the enrichment of ATAC-seq around the human oocyte PMDs in human early embryos and TBEs. The upstream and downstream regions are 1 × PMD length away from the PMD boundary. f, The average enrichment of ATAC-seq around the oocyte PMDs in human early embryos and TBEs. The upstream and downstream regions are 1 × PMD length away from the PMD boundary.