Extended Data Fig. 7: HRF–MS of LPS–EcMsbA in the presence and absence of G907 and G092.
From: Structural basis for dual-mode inhibition of the ABC transporter MsbA
a, A schematic overview (with the target protein illustrated as a cartoon IgG shown in green) of the HRF–MS experiments performed in this study on LPS–EcMsbA in FA-3 detergent conditions in the presence or absence of G907 and G092. LC–MS/MS, liquid chromatography with tandem mass spectrometry; UPLC, ultra-performance liquid chromatography. b–c, Data are displayed as the differences measured in tryptic peptide oxidation of the ligand-bound condition with G907 present (b) or G092 present (c) relative to the inhibitor-free LPS–EcMsbA solution sample. The median residue number of the individual tryptic peptides (summarized in Extended Data Table 2 for the G907 experiment) is indicated on the x-axis. For both HRF–MS analyses, data are mean ± s.e.m. from three replicate experiments. d, A cartoon rendering of the G907–LPS–EcMsbA crystal structure coloured according to the measured differences in tryptic peptide oxidation between the inhibitor-free and G907-bound condition presented in b. Regions without significant measured changes are coloured in dark grey and are found mainly within the transmembrane region; regions lacking robust peptide coverage are coloured in light grey. The phospho-N-acetylglucosamine moieties of the bound LPS molecule are shown only for reference, the remainder of LPS and G907 are omitted for clarity.
