Extended Data Fig. 9: Oligodendrocyte environment generates the GCI-α-Syn strain.

a, Immunohistochemistry of adjacent sections from human or mouse brains with Syn303 and Syn7015. First row, adjacent brain sections of cases of Lewy body disease and MSA used for the extraction of LB-α-Syn and GCI-α-Syn for injection. Second row, adjacent brain sections of KOM2 mice injected with LB-α-Syn prepared from the brain tissue shown in the first row. OPT and CP are shown. Whereas the LB-α-Syn used for the injections is Syn7015-negative, the oligodendrocyte pathology that is induced is Syn7015-positive. Third row, adjacent brain sections of KOM2 mice injected with GCI-α-Syn prepared from the brain sample shown in the first row. OPT and CP are shown. Fourth row, adjacent brain sections of M83 mice with α-Syn pathology. Midbrain (MB) and pons are shown. b, Brain sections from KOM2 mice injected with GCI-α-Syn or LB-α-Syn in the thalamus were double-labelled with Syn506 and antibodies against P62 (left panel) or ubiquitin (right panel). c, Brain sections from GCI-α-Syn- or LB-α-Syn-injected KOM2 mice were double-labelled with Syn506 and GFAP. Both ipsilateral and contralateral optic tracts are shown. d, Adjacent sections of substantia nigra and cortex from two different cases of MSA were stained with Syn7015 and Syn303. Scale bars: 50 μm (a–c), 12.5 μm (a insets), 20 μm (b inset) and 30 μm (d). The experiments in a–d have been repeated three times with similar results.