Extended Data Fig. 2: Syn7015 preferentially recognizes GCIs over Lewy bodies.
From: Cellular milieu imparts distinct pathological α-synuclein strains in α-synucleinopathies
a, Immunohistochemistry using a series dilution of Syn303 or Syn7015 on serial sections of a DLB brain and a MSA brain. At 45 ng ml−1, Syn7015 recognized both Lewy bodies and GCIs. At lower concentrations—particularly 1.67 ng ml−1 and 0.56 ng ml−1—Syn7015 preferentially recognizes GCIs over Lewy bodies (repeated with four cases). b, Quantification of the area occupied by pathological α-Syn stained with serial dilutions of Syn7015 or Syn303 on serial sections of two cases of MSA-P, two cases of MSA-C, one case of AD, two cases of PDD and two cases of DLB. The results for each case are normalized to Syn303 staining at 45 ng ml−1 (GCI, n = 4 cases; LB, n = 5 cases). c, α-Syn pathology revealed by Syn303 or Syn7015 in adjacent sections from cases of Lewy body disease and MSA (repeated with seven cases). Results shown as mean ± s.e.m. *P < 0.05. Scale bar: 50 μm (a), 100 μm (c), 25 μm (c inset). See Supplementary Table 5 for statistical details.
