Extended Data Fig. 7: Gene-editing of MXRA8 in human cell lines.
From: Mxra8 is a receptor for multiple arthritogenic alphaviruses
a, Flow cytometry analysis of MXRA8 expression in human MRC-5, HFF-1, RPE and Hs 633T cells expressing control or two different MXRA8 sgRNAs. Data are representative of two experiments. b, Gene-edited cells were inoculated with CHIKV (strains 181/25, AF15561 or LR-2006) in HFF-1, RPE, and Hs 633T cells. Cells were stained for viral antigen with an anti-E2 antibody. Data are pooled from two (HFF-1 and Hs 633T) or three (RPE) independent experiments and expressed as mean values ± s.d. (n = 6; one-way ANOVA with a Dunnett’s multiple comparison test compared to the control). ****P < 0.0001.
