Extended Data Fig. 10: Binding of Mxra8–Fc to surface-displayed E2 protein in virus-infected cells and mapping of Mxra8 binding site on E2.
From: Mxra8 is a receptor for multiple arthritogenic alphaviruses
a, Diagram of the cell-based binding assay. After infection, viral structural proteins (for example, E2) traffic to the cell plasma membrane where progeny virion assembles and buds. E2 protein is displayed on the cell surface and is accessible to the binding of Mxra8–Fc and detection with a goat anti-mouse IgG secondary antibody by flow cytometry. b, Binding of Mxra8–Fc to virus-infected wild-type 3T3 cells. Cell were infected with the indicated viruses and processed for Mxra8–Fc binding by flow cytometry. Virus-specific anti-E2 antibodies were used as positive controls. Data are representative of two independent experiments. c, d, Mapped residues are shown as magenta spheres (c) or sticks (d) on the CHIKV p62–E1 structure (c, trimer of dimers, top view; d, heterodimer, side view) using PyMOL (Protein Data Bank code: 3N41). The E1 and E2 proteins are coloured in grey and cyan, respectively.
