Extended Data Fig. 7: PGA1_c00920 partially rescues the glycine growth defect of an E. coli cycA mutant.
From: Mutant phenotypes for thousands of bacterial genes of unknown function
CycA is a glycine transporter from E. coli and a mutant in this gene has reduced uptake of glycine37. We investigated whether a member of the UPF0126 protein family could rescue the glycine growth defect of an E. coli cycA deletion strain. We introduced different plasmids into the E. coli cycA Keio collection deletion background: an empty plasmid with no insert (cycA− + empty), a plasmid with a wild-type allele of the E. coli cycA gene (cycA− + cycA), and a plasmid with PGA1_c00920 from P. inhibens (cycA− + PGA1_c00920). We compared the growth of these strains and a wild-type E. coli control (wt + empty) in defined media with either ammonium chloride (n = 2 independent experiments per strain) or glycine as the sole source of nitrogen (n = 4 independent experiments per strain). PGA1_c00920 partially rescues the glycine-specific growth defect of the cycA− deletion strain.
