Extended Data Fig. 10: Alternative ways of computing cofitness.
From: Mutant phenotypes for thousands of bacterial genes of unknown function
a, The effect of rescaling the cofitness values by the number of generations in six bacteria. For each of the six bacteria, we identified all pairs of protein-coding genes that were assigned to the same TIGR subrole, were more than 20 kB apart, and had fitness data. This gave 1,711–9,406 pairs per bacterium. We also selected a random subset of pairs that were assigned to different TIGR subroles, were more than 20 kB apart, and had fitness data (1,559–8,881 pairs per bacterium). For each pair, we compared the original cofitness values to the rescaled cofitness (computed from fitness values that were divided by the number of generations). b, The effect of averaging fitness scores from replicate experiments on the cofitness values.
