Extended Data Fig. 6: Microbial signals are sufficient for PMP in Tet2−/− mice.
From: Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host
a, TLR2 activation measured using a HEK-TLR2 reporter assay (n = 5 biological replicates). b, Schematic of Pam3CSK4 in vivo treatment of symptom-free Tet2f/fVavcre mice that are over 20 weeks old, and littermate controls. c, Numbers of CD11b+ monocytes prior (day 0), during treatment (day 2) and at end point analysis (day 14) (n = 6 mice). Mean ± s.e.m. d, Percentage of CD11b+Gr1+ myeloid cells (left, n = 5 (Tet2f/fcre−, no Pam3CSK4), 6 (Tet2f/fcre−, with Pam3CSK4), 7 (Tet2f/fVavcre, no Pam3CSK4) or 6 (Tet2f/fVavcre, with Pam3CSK4) mice) and numbers of GMP cells (right, n = 6 (Tet2f/fcre−, no Pam3CSK4), 6 (Tet2f/fcre−, with Pam3CSK4), 7 (Tet2f/fVavcre, no Pam3CSK4) or 6 (Tet2f/fVavcre, with Pam3CSK4) mice). e, Intestinal permeability measurement by blood plasma FITC–dextran concentrations at end point analysis (n = 6 mice in all cases). a, c–e, Centre is mean. a, d, One-way ANOVA, Sidak’s post hoc test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are representative of at least two independent experiments.
