Extended Data Fig. 7: Skewed TCR repertoire between CD39⁻ and CD39⁺ CD8⁺ TILs.

To further explore the specificity of CD39⁺ CD8⁺ TILs, we performed TCRα and TCRβ sequencing of CD39⁻ and CD39⁺ CD8⁺ TILs. We assume that a less diverse TCRα or TCRβ profile in CD39⁺ CD8⁺ TILs would suggest tumour antigen-driven clonal expansion, as suggested²³. a, The clonality index, incorporating the frequency of each unique TCRα or TCRβ clone in paired samples (n = 8 patients), indicated a lower TCRα and TCRβ diversity in CD39⁺ CD8⁺ TILs. Two-tailed paired t-test. Data are from two independent experiments. b, c, We also compared TCRα repertoires between these populations and found that the most highly represented clones were not shared between CD39⁻ and CD39⁺ CD8⁺ TILs. Taken together, the less clonal and skewed TCRα profile of CD39⁺ CD8⁺ TILs supports the notion that these cells underwent tumour antigen-driven clonal expansion. Data are from two independent experiments.